Medicinal Plants

Title: Investigation on the antioxidant activity of Mentha aquatica, Curcuma longa, Embilica officinalis, Glycyrrhiza glabra and Nigella sativa Name of the student:Ali Abbas Abstract

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Extracts of five different medicinal plants [Mentha aquatica (Stem and Leaves), Curcuma longa (Stem), Embilica officinalis (Fruits), Glycyrrhiza glabra (Stem) and Nigella sativa (Seeds)]in two different solvents: 80% methanol and 80% ethanol (solvent : water 80:20 v/v) were investigated for total phenolics (TP), total flavonoids (TF) and their antioxidant activity (AA) by measuring reducing power, inhibition of peroxidation using linoleic acid system and 2,2/-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity.

A large difference was observed in all TP, TF, inhibition of linoleic acid oxidation, DPPH scavenging activity and reducing power of different plant extracts. Total phenolic contents of all plant extracts ranged from 13. 87-28. 63 gallic acid equivalents and total flavonoid contents ranged from 0. 96-16. 90 catechin equivalents. Reducing power at 10mg/mL extract concentration ranged from 0. 983-1. 527. All the plant extracts inhibited oxidation of linoleic acid by 35. 90-71. 9%, while DPPH scavenging activity ranged from 39. 55-80. 55%. Extraction efficiency of components with antioxidant property is greater in ethanol extract. Mentha aquatica has the highest amount of TP ranged from 25. 06-28. 63 g/100g, while the highest amount of TF was observed in Embilica officinalis and the same had the highest DPPH scavenging activity. The highest AA measured by inhibition of linoleic acid oxidation is offered by Glycyrrhiza galbra and the highest reducing power is observed for Nigella sativa.

Title: Investigation on antioxidant activity of Mehndi, Kasni, Harmal and Kori booti. Name:Javed Iqbal Abstract Extracts of four different medicinal plants like [Alkanna tinctoria (Stem and Leaves), Cichorium intybus (Roots), Peganum harmala (Seeds) and Ajuga bractiosa (Stem and leaves)] in two different solvents: 80% ethanol and 80% methanol (Solvent : water, 80:20 v/v) were evaluated for their antioxidant activity (AA), total phenolics (TP) and total flavonoid contents (TF).

Antioxidant activity was determined by measuring reducing power, inhibition of peroxidation using linoleic acid and 2,2/-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. A wide range of differences were observed in TP, TF, inhibition of linoleic acid oxidation and DPPH scavenging activity of all medicinal plant extracts. All plant extracts exhibited a wide range of TP 9. 87-34. 79 gallic acid equivalent and total flavonoid contents 1. 80-6. 55 catechin equivalent. Reducing power at 10mg/mL extract concentration ranged from 0. 827 to 1. 627.

Different plant extracts inhibited oxidation of linoleic acid by 25. 64-64. 10%, while DPPH radical scavenging activity ranged from 54. 45% to 81. 73%. Ethanol has best extraction efficiency of components with antioxidant activity than methanol. Ajuga bractiosa has the highest amount of TP ranged from 16. 18-34. 79 g/100g and the same has the highest antioxidant activity in linoleic acid system. The highest amount of TF was observed in Peganum harmala while Cichorium intybus had the highest DPPH scavenging activity and the same had the highest reducing power.

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